Which is the correct order for you to perform these steps in order to sequence the new species’ genome?

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You wish to perform whole-genome shotgun sequencing of a new species of yeast that you have identified growing in the spine of your Genetics textbook. Consider the following steps that you would perform.

1. Mix genomic DNA fragments from the yeast with a plasmid vector digested with BamHI and add DNA ligase.

2. Isolate DNA from bacterial colonies and carry out dideoxy sequencing on each of them.

3. Use a computer to ‘assemble’ the DNA sequences into the order they were in the intact genome.

4. Isolate genomic DNA from the organism and digest it with a restriction enzyme such as BamHI.

5. Transform the DNA mixture into bacteria and plate on plates containing ampicillin (assume the vector carries ampicillin resistance gene).

a) 4 - 1 - 2 - 5 - 3

b) 4 - 1 - 5 - 3 - 2

c) 4 - 1 - 5 - 2 - 3

d) 4 - 5 - 1 - 2 - 3

e ) 4 - 5 - 1 - 3 - 2
asked May 4, 2016 in Genetics by georgepak112 ~Rookie~ (75 points)
    

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